FIG. 1.

Generation of ALX-deficient mice via homologous recombination. (A) Schematic of the targeting construct designed to delete exons 2 and 3 via homologous recombination. H3, HindIII; TK, herpes simplex virus thymidine kinase; wt, wild type; IRES, internal ribosome entry site; GFP, green fluorescent protein; DT, diphtheria toxin. (B) Southern blot depicting targeted ES cell clones digested with HindIII and detected with a probe from the last exon of ALX, which is outside of the targeting construct (shown in panel A). All clones had the expected endogenous band (11.6 kb), while clone #83 also possessed a targeted allele (6.1 kb). (C) Western blot (WB) of wild-type (WT), heterozygous (Het), and ALX-deficient (KO) splenocytes probed for expression of ALX protein. Each lane contained lysates from the same number of cell equivalents.