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. 1999 Mar 16;96(6):3251–3256. doi: 10.1073/pnas.96.6.3251

Figure 1.

Figure 1

(A) Western blot analysis of SOD from PC12 cells 3 days after mock infection (lane 1) or infection with a control AdLacZ (lane 2), AdSODWT (lane 3), AdSODA4V (lane 4), or AdSODV148G (lane 5). Cell lysates were subjected to SDS/PAGE, were blotted onto nitrocellulose, and then were incubated with an anti-SOD polyclonal antibody. Note the immunoreactivity in all lanes of a protein corresponding to the electrophoretic mobility of the rodent form of SOD (R-SOD) at ≈19 kDa (lower band). In the case of infection with AdSODWT, AdSODA4V, and AdSODV148G (lanes 3–5, respectively), an immunostained protein at ≈21 kDa (upper band) is seen, corresponding to the electrophoretic mobility of human SOD (H-SOD). (B) Cell death of differentiated PC12 cells 5 days after infection with mock, AdSODWT AdSODV148G, or AdSODA4V. Expression of SODV148G and SODA4V caused cell death whereas there was no significant alteration in the viability of control cells or SODWT-expressing cells.