Figure 2.

Assessment of CsA influence on calcineurin activity and immunoblot analysis of calcineurin at a time before evidence of apoptotic cell loss. (A) Calcineurin activity was assayed 3 days after AdV infection or 1 day after NGF withdrawal. Cells expressing SODWT exhibited higher calcineurin activity than mock-infected cells, cells expressing SODV148G, or after NGF withdrawal. (B) Treatment with CsA (1 μM) dramatically decreased calcineurin activity in all cases. (C) Similar immunostaining of calcineurin is seen in Western blot analysis after mock (lane 1), NGF removal (lane 2), SODV148G (lane 3), or SODWT expression (lane 4).