FIG. 3.

Activation of S100A9, S100A7, JAG2, and CCND1 promoters in response to GLI and EGF signaling. RLU, relative light units; cont, control. (A) Illustration of the location of Gli binding sites in the promoter region of S100A9, S100A7, JAG2, and CCND1. Arrows mark the positions of forward and reverse PCR primers used for cloning of the corresponding luciferase reporter constructs. (B) Reporter assays showing activation of luciferase activity in response to GLI1 and GLI2* in 293 cells. (C) Enhanced induction of S100A7 and S100A9 but not of PTCH reporter activity by combinatorial GLI1 (GLI2*)/EGF signaling in HaCaT keratinocytes.