TABLE 1.
qRT-PCR analysis of GLI1/EGF-regulated target gene expression in human keratinocytes
| HUGO gene designation | Basis for nomenclaturea | NCBI accession no. | Fold mRNA induction change compared to untreated controls (SD)
|
||
|---|---|---|---|---|---|
| EGF | GLI1 | GLI1/EGF | |||
| Class I (GLI1/EGF > GLI1 + EGF) | |||||
| EGR3 | Early growth response 3 | NM_004430 | 7.7 (2.1) | 1.2 (0.3) | 652.6 (192.2) |
| S100A7 | S100 calcium binding protein A7 (psoriasin 1) | NM_002963 | 2.3 (0.6) | 9.8 (2.5) | 1,351.1 (156.3) |
| IL1R2 | Interleukin-1 receptor, type II | NM_004633 | 2.4 (0.3) | 9.9 (0.4) | 999.5 (165.3) |
| S100A9 | S100 calcium binding protein A9 (calgranulin B) | NM_002965 | 1.2 (0.2) | −1.6 (0.3) | 24.3 (3.2) |
| FOXA2 | Forkhead box A2 | NM_021784 | 76.1 (12.9) | 14.4 (2.4) | 494.6 (65.6) |
| ARC | Activity-regulated cytoskeleton-associated protein | NM_015193 | 1.7 (0.4) | 1,097.5 (98.4) | 11,585.2 (967.3) |
| LNK | Lymphocyte specific adapter protein Lnk (signal transduction protein Lnk) (lymphocyte adapter protein) | NM_005475 | 2.5 (0.5) | 1.5 (0.4) | 9.2 (0.9) |
| ID2 | Inhibitor of DNA binding 2, dominant negative helix-loop-helix protein | NM_002166 | 1.7 (0.6) | 6.5 (0.9) | 33.1 (4.9) |
| JAG2 | Jagged 2 | NM_002226 | 1.9 (0.3) | 19.0 (2.4) | 90.5 (11.3) |
| MAPK11 | Mitogen-activated protein kinase 11 | NM_002751 | −1.1 (0.2) | −2 (0.7) | 2.1 (0.2) |
| TUFM | Tu translation elongation factor, mitochondrial | NM_003321 | 1.3 (0.2) | −1.2 (0.3) | 2.9 (0.4) |
| PHLDA2 | Pleckstrin homology-like domain, family A, member 2 | NM_003311 | 2.7 (0.4) | 5.3 (0.4) | 17.1 (1.7) |
| IDS | Iduronate 2-sulfatase (Hunter syndrome) | NM_000202 | 1.9 (0.2) | 2.2 (0.1) | 7.0 (0.4) |
| CDC34 | Cell division cycle 34 | NM_004359 | 1.0 (0.2) | 3.7 (0.2) | 10.6 (1.7) |
| ULK1 | Unc-51-like kinase 1 (C. elegans) | NM_003565 | −1.4 (0.3) | 8.3 (1.6) | 21.9 (2.1) |
| CCND1 | Cyclin D1 | NM_053056 | 1.5 (0.1) | 2.5 (0.3) | 5.1 (0.6) |
| RXRA | Retinoid X receptor, alpha | NM_002957 | −1.1 (0.3) | 6.1 (0.8) | 11.3 (1.9) |
| CDH1 | Cadherin 1, type 1, E-cadherin (epithelial) | NM_004360 | 1.0 (0.3) | 1.9 (0.2) | 3.5 (0.4) |
| RPS6KA1 | Ribosomal protein S6 kinase, 90-kDa, polypeptide 1 | NM_002953 | 1.2 (0.4) | 1.1 (0.1) | 1.8 (0.1) |
| Class II (GLI1/ EGF ∼ GLI) | |||||
| PTCH | Patched homolog (Drosophila) | NM_000264 | −2.1 (0.3) | 29.9 (3.1) | 19.7 (2.7) |
| BCL2 | B-cell CLL/lymphoma 2 | NM_000633 | 2.2 (0.4) | 7.2 (0.9) | 7.7 (0.6) |
| CTSL | Cathepsin L | NM_001912 | 1.3 (0.2) | 12.7 (2.0) | 8.8 (1.4) |
| TCEA2 | Transcription elongation factor A (SII) 2 | NM_198723 | −1.6 (0.2) | 123.6 (12.1) | 119.4 (17.6) |
| FYN | FYN oncogene protein related to SRC, FGR, YES | NM_002037 | 1.2 (0.2) | 4.8 (0.3) | 8.3 (0.5) |
| DGCR6 | DiGeorge syndrome critical region gene 6 protein | NM_005675 | −1.2 (0.2) | 2.3 (0.1) | 2.3 (0.2) |
| PITRM1 | Pitrilysin metallopeptidase 1 | NM_014889 | 1.5 (0.1) | 3.7 (0.4) | 2.4 (0.5) |
| MYLK | Myosin, light polypeptide kinase | NM_053025 | −2.2 (0.7) | 5.1 (1.5) | 3.6 (1.4) |
| MNAT1 | Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) | NM_002431 | 1.4 (0.4) | 1.7 (0.2) | 2.1 (0.3) |
| MYC | v-myc myelocytomatosis viral oncogene homolog protein (avian) | NM_002467 | 1 (0.2) | −4.3 (0.6) | −2.5 (0.2) |
| BNC1 | Basonuclin 1 | NM_001717 | 1.8 (0.2) | −2.8 (0.14) | −2.4 (0.3) |
a
Nomenclature is per the HUGO database (approved gene names).