FIG. 14.

Intranuclear redistribution of endogenous N-CoR by DHT-bound AR and release of the endogenous N-CoR from the AR compartment by exogenously expressed coactivators. 3T3-L1 cells were treated with ethanol (Ai) or 10 nM DHT (Aii) for 1 h and then subjected to immunofluorescence staining using anti-N-CoR antibody. 3T3-L1 cells were transfected with the expression plasmids for AR-GFP with empty vector (B and C), with nonfluorescent SRC-1 (D), or with nonfluorescent CBP (E). The molar ratio of transfected amounts of expression plasmids for two proteins was 1:3. Twenty-four hours after the transfection, the cells were subjected to immunofluorescence staining in the absence (B) or presence (C, D, and E) of 10 nM DHT, and then images were collected. Signals from AR-GFP (green, i)- and Alexa Fluor 546 (red, ii)-labeled endogenous N-CoR were obtained by laser confocal microscopy, and these two signals were merged (iii). The area indicated in the white rectangle in panel iii is magnified in panel iv. The white arrows indicate the intranuclear discrete dots in red, which were derived from the labeled endogenous N-CoR proteins. Bars, 5 μm.