FIG. 5.

The middle region of the N-CoR is responsible for the redistribution by agonist-bound steroid hormone receptors. COS-7 cells were cotransfected with expression plasmids for GFP-tagged full-length N-CoR or its truncated mutants and nonfluorescent wild-type AR (panels A and B), GRα (panels C and D), or ERα (panels E and F). The cells in panels A, C, and E were observed in the absence of ligands, and the cells were incubated with 10 nM DHT (panel B), 100 nM Dex (panel D), or 1 μM E₂ (panel F) for 1 h at 37°C before images were taken. The molar ratios of transfected amounts of expression plasmids for GFP-N-CoR and its mutants to steroid hormone receptors were 3:1 in all of the transfection experiments. Bars, 5 μm.