FIG. 6.

The middle region of the N-CoR plays a major role in suppressing ligand-dependent transactivation function of steroid hormone receptors. COS-7 cells were cotransfected with expression plasmids for full-length N-CoR or its mutants and AR (A), GRα (B) or ERα (C), with reporter plasmids carrying each hormone responsive element and pRL-CMV. These cells were cultured in the presence (+) or absence (−) of the agonists for 24 h and subjected to the luciferase assay. The luciferase activities are represented as values relative to the activity induced by the full-length steroid hormone receptor alone in the presence of each ligand. Each bar represents the mean ± standard deviation of results from three independent experiments. *, P < 0.05.