FIG. 7.

Multiple regions of the N-CoR are involved in the interaction with DHT-bound AR. (A to C) Coimmunoprecipitation of the full-length and truncated N-CoR with DHT-bound AR. COS-7 cells were cotransfected with expression plasmids for the wild-type AR and GFP-fused full-length N-CoR or its truncated mutants, VP16 or VP16-fused N-CoR mutants, or YFP or YFP-fused N-CoR mutants, as indicated. After 24 h incubation with 10 nM DHT, the cells were lysed and immunoprecipitation (IP) was performed using antibodies against AR, GFP, or VP16, as indicated. The precipitates were then subjected to Western blotting (WB) analysis using antibodies as indicated. +, present; −, absent.