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. 2006 Sep;26(17):6453–6468. doi: 10.1128/MCB.00356-06

FIG. 3.

FIG. 3.

A BAC-based RANKL transcriptional reporter construct is responsive to PTH. (A) UAMS-32P cells were stably transfected with the RANKL 2-kb reporter construct (RANKL 2kb) or a reporter construct derived from a BAC containing the entire murine RANKL gene as well as 120 kb of 5′-flanking region and 14 kb of 3′-flanking region (BAC1). The BAC1 construct is depicted graphically with the relative positions of exons indicated by boxes and the RANKL coding region indicated by filled boxes. The sizes of the introns and exons are not drawn to scale. Pools of transfected cells were treated with vehicle (veh), 10−7 M PTH, 1.5 mM db-cAMP, 25 ng/ml OSM, or 10−8 M 1,25(OH)2D3 for 24 h, and relative light units (RLU) were determined. (B) UAMS-32P, Hepa, or RAW264.7 cells stably transfected with the BAC1 reporter construct were treated with 1.5 mM db-cAMP for 24 h, and RLU were determined. (C) UAMS-32P, Hepa, or RAW264.7 cells stably transfected with a reporter construct containing three cAMP response elements upstream from a minimal promoter (3×CRE), were treated with db-cAMP as described for panel B. The values shown in each panel are the mean ± SD of triplicate wells.