Skip to main content
. 2006 Sep;26(17):6395–6402. doi: 10.1128/MCB.00723-06

FIG. 5.

FIG. 5.

The ELM2 domain of CoREST confers nucleosomal deacetylation. (A) Map of five mammalian expression constructs for CoREST and its mutants containing FLAG tag (ΔELM2, ΔSANT2, ΔSANT1, and ELM2). All CoREST constructs lack 30 amino acids from position 31 to 60. (B) Western blot analysis of BHC110 and HDAC2 coimmunoprecipitated with CoREST and its mutants. HEK-293 cells were transfected with CoREST and its mutants, and whole-cell extracts were immunoprecipitated with anti-FLAG M2 resin. The eluates were analyzed by Western blotting using anti-BHC110, anti-HDAC2, and anti-FLAG antibodies. (C) Effect of ELM2 domain on nucleosomal deacetylation. The amounts of recombinant proteins used were approximately as follows: rBHC110, 800 ng (+) and 1600 ng (++); rHDAC1, 400 ng (+); rCoREST, 400 ng (+); rELM2, 150 ng (+). Nucleosomes were used as substrates, and reaction mixtures were analyzed by SDS-PAGE, followed by Western blotting.