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. 2006 Sep;26(17):6395–6402. doi: 10.1128/MCB.00723-06

FIG. 6.

FIG. 6.

Deacetylation and demethylation are functionally cooperative in vivo. (A) Analysis of dimethyl K4 H3 levels on the synapsin promoter by quantitative chromatin immunoprecipitation (ChIP) assay after transient transfection of HEK 293 cells with plasmids encoding BHC110 or its mutant (K661A). (B) Analysis of acetyl H3 levels on synapsin promoter by quantitative ChIP assay after transient transfection of HEK 293 cells with plasmids encoding BHC110 or its mutant (K661A). (C) Analysis of dimethyl K4/acetyl H3 levels on synapsin promoter by quantitative ChIP assay after trichostatin A treatment (100 ng/ml) of HEK 293 cells.