FIG. 5.

G-overhang maintenance in ER-POT1 cells. (A to C) In-gel hybridization of (TA₂C₃)₄ G-strand probe to MspI-, HinfI-, and HaeIII-digested DNA from ER-POT1 cells. (A) ER-POT1 clone 37 grown with (+) or without (−) tamoxifen (Tam) for 4, 8, 12, 16, 20, or 24 h. (B) Samples were treated with Exo1 or mung bean nuclease (MBean) prior to restriction digestion. (C) Gels were hybridized with telomeric G-strand or C-strand probe. (D) Histogram showing relative G-overhang signals for the experiment shown in panel A. The signal in each lane was quantified with a phosphorimager and normalized for loading. The average normalized signal for the +Tam lanes was set to 100%. (E) Histogram showing the G-overhang signals 24 h after tamoxifen removal for ER-POT1 clones 16, 17, and 37. Values are means from three or more independent experiments, and error bars show standard deviations.