FIG. 7.

Defects in chromosome segregation and centrosome amplification. Caffeine and nocodazole were added to ER-POT1 cells grown with or without tamoxifen (+Tam or −Tam, respectively) for 20 h. The nocodazole was removed after 4 h, and cells were fixed 60 min (A and B) or 120 min (C) later. (A) DAPI and phospho-H3 staining showing delayed anaphase and abnormal mitotic figures. Panels i and ii and iii and iv are matched pairs. Arrows indicate anaphase cells, and arrowheads indicate abnormal mitotic figures. (B) γ-Tubulin staining showing centrosome amplification. (C) DAPI staining showing abnormal nuclei following missegregation or mitotic catastrophe.