TABLE 1.

Abundance of hY RNAs in HeLa cell S100 extracts

Sample RNA	Mean ± SD
	−ΔCTa	Relative RNA amount (103)b	RNA concnc
HPRT mRNA	0	1	0.8 pM
hY1 RNA	14.1 ± 0.5	17.6 ± 7.3	13.4 ± 5.6 nM
hY3 RNA	14.95 ± 0.36	31.7 ± 8.7	24.2 ± 6.6 nM
hY4 RNA	13.6 ± 0.76	12.4 ± 8.6	9.5 ± 6.6 nM
hY5 RNA	16.67 ± 0.47	104.3 ± 40.2	79.4 ± 30.7 nM
5S rRNA	17.26 ± 0.44	157 ± 56	120 ± 73.4 nM

^a−ΔC_T values were obtained as specified in Materials and Methods as averages from seven acquisition runs for each cDNA prepared from an S100 extract (as a reference, the protein concentration of the extract was 10 mg/ml). HPRT mRNA was used as reference RNA. Mean values ± standard deviations of the means from two independent experiments using two different S100 preparations are shown.

^bRNA amounts relative to the HPRT reference were calculated from the ΔC_T values as specified in Materials and Methods.

^cThe RNA concentration in the S100 extract was calculated from the relative RNA amounts by using calibration curves obtained by real-time PCR amplifications of defined amounts of hY cDNAs. The conversion factor of 0.763 × 10⁻³ nM was obtained as the average from three independent calibrations.