FIG. 6.

Participation of PI3K in TSA-induced LHR gene activation. (A and B) Reporter gene analyses of LHR gene promoter activity in JAR cells that were pretreated with wortmannin or LY294002 for 1 h, followed by incubation with or without TSA (100 ng/ml) for 24 h. (C) Reporter gene analyses of LHR gene promoter activity in JAR cells in the presence of a DN PI3K p110α mutant construct. Cells were treated with or without TSA for 24 h prior to analyses. Relative luciferase activities are indicated as n-fold induction over enzyme activity in the absence of drug treatment or the PI3K p110α DN mutant form.