TABLE 1.
List of constructs used in this study
| Construct name | Description | Reference or source |
|---|---|---|
| Cμ | Wild-type gene | 32 |
| pA11 | 11 nt surrounding AAUAAA are deleted | This work |
| pA21 | 21 nt surrounding AAUAAA are deleted | This work |
| pA1 | 5 nt surrounding AAUAAA are mutated | This work |
| pA2 | 19 nt surrounding AAUAAA are mutated away from AU richness | This work |
| ΔU | Distal GU-rich element is mutated | 29 |
| ΔU1 | Proximal GU-rich element is mutated | This work |
| ΔU2 | Both GU-rich elements are mutated | This work |
| ΔU2pt | Same as ΔU2, but with a spurious point mutation | This work |
| GU3 | New GU-rich element inserted between the two natural GU-rich elements | This work |
| ΔUGU | New GU-rich element inserted into ΔU | This work |
| ΔU1GU | New GU-rich element inserted into ΔU1 | This work |
| ΔU2ptGU | New GU-rich element inserted into ΔU2pt | This work |
| ΔNH | NotI-HindIII fragment containing the pause site deleted | 29 |
| ΔU2ΔNH | Pause site deleted from ΔU2 | This work |
| ΔUGUΔNH | Pause site deleted from ΔUGU | This work |
| ΔU2ptGUΔNH | pause site deleted from ΔU2ptGU | This work |
| OCTA | 7 nt located 40 nt upstream from AAUAAA are mutated | This work |
| αs-m | μs poly(A) signal replaced with αs poly(A) signal | 31 |