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. 2006 Sep;26(18):6772–6785. doi: 10.1128/MCB.00342-06

FIG. 4.

FIG. 4.

Nup214 and Nup358 are not required for nuclear protein import. Nup214- or Nup358-depleted HeLa cells were transiently transfected with constructs coding for GFP-GFP-NLS (A) or GFP-GFP-M9 (B), stained for Nup214 and Nup358, and analyzed by fluorescence microscopy. (C) Mock-treated or Nup214- or Nup358-depleted HeLa-GFP-NFAT cells were fixed before (−ionomycin) or after (+ionomycin) treatment with ionomycin, stained for residual nucleoporins at the NPC, and analyzed by fluorescence microscopy. For a better comparison of RNAi and transport efficiencies, nucleoporin-depleted HeLa-GFP-NFAT cells were mixed with untransfected cells. (A to C) Antibodies used for detection of Nup214 or Nup358 are indicated in the individual pictures (right panels).