FIG. 1.

ChIP-chip analysis reveals ER-GATA-1 occupancy far upstream of Gata2. Gata2 locus organization is illustrated at the top. Open and filled boxes depict noncoding and coding exons, respectively. Arrows pointing down depict GATA switch sites and the −77 kb region. The VISTA plot depicts percent sequence identity between mouse and human (M/H) or mouse and dog (M/D) as a function of genomic coordinates. The first three ChIP-chip profiles depict results comparing chromatin immunoprecipitation with anti-GATA-1 and preimmune antibodies. The bottom three ChIP-chip profiles compare signals obtained upon immunoprecipitation with anti-GATA-1 antibody with that of input chromatin. Note that no qualitative differences were detected in the six ChIP-chip profiles. These results are representative of those obtained from three independent ChIP-chip experiments. The “peaks” delineated with Signal Map software represent the highest signal/background ratios obtained using default software settings.