FIG. 2.

Representative ChIP experiment used for CGI microarray hybridization. (A) Representative ChIP experiment used for CGI array experiments. HCT116 p53^+/+ cells were exposed to hypoxia (H), 0.3 μg/ml adriamycin (A), or control conditions (N) for 12 h. PCR against the p21 p53RE was used to verify specific enrichment. (B) Verification of DNA amplification after ligation-mediated PCR (LM-PCR). Approximately 10% of amplified DNA was electrophoresed on a 1.5% agarose gel alongside a 1-kb DNA ladder. All amplicons are approximately 600 bp in length. (C) PCR for p21 p53RE after amplification. Ten nanograms of LM-PCR amplicon was added to a PCR using primers specific for the p21 p53RE. For all figures, IgG refers to samples derived for the IP-negative control, p53 refers to DNA immunoprecipitated with DO-1 antibody, and input refers to the DNA derived from samples prior to immunoprecipitation.