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. 2006 Oct;26(19):7130–7144. doi: 10.1128/MCB.00331-06

FIG. 5.

FIG. 5.

ADAP/SKAP55 complex can signal in the nonraft fraction of the plasma membrane. (A) Scheme showing the individual LAT-SKAP55 and LAT-ADAP chimeras used in these experiments. SXXS mutants carry cysteine-to-serine mutations at positions 26 and 29 of LAT. EC, extracellular domain; TM, transmembrane domain; y, tyrosine residue. (B) Jurkat T cells transiently overexpressing the LAT-SKAP55 chimeras were left unstimulated (non) or were stimulated with CD3 MAb OKT3 (TCR) for 30 min and then analyzed for adhesion to fibronectin or ICAM-1. The expression of the individual SKAP55 mutants was assessed by anti-SKAP55 Western blotting. Data represent the means and SE of at least three independent experiments. (C) Jurkat T cells transiently overexpressing the LAT-ADAP chimeras were either left unstimulated (non) or stimulated for 30 min with CD3 MAb OKT3 (TCR) and subsequently analyzed for adhesion to fibronectin or ICAM-1. The expression of the ADAP mutants was analyzed by anti-ADAP Western blotting. Data represent the means and SE of three independent experiments.