Expression of M-Ras in mouse tissues. (A) The specificity of M-Ras antibody, R3C18, was tested with lysates from 293T cells transfected with H-Ras, R-Ras, TC21, and M-Ras expression plasmids. Western blot analysis was performed with antibodies raised against individual G proteins. The anti-TC21 antibody cross-reacted with H-Ras and R-Ras. (B) The expression of Ras-related proteins in various mouse organs was analyzed by Western blot analysis with the corresponding antibodies. Note the high level of M-Ras expression in the brain.