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. 2006 Oct;26(19):7145–7154. doi: 10.1128/MCB.00476-06

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Copyright © 2006, American Society for Microbiology

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FIG. 2. — Expression of M-Ras in the CNS. (A) The expression of M-Ras in microdissected hippocampi from different embryonic stages was analyzed. Cell extracts from DIV cultures of cortical (B) and hippocampal (C) neurons were analyzed for M-Ras expression. Total cell extracts derived from adult brain (A) were used as a positive control. (D) A total brain lysate and primary cultures of cortical neurons (CN), astrocytes (AST), and oligodendrocytes (OL) were analyzed for the expression of both M-Ras and H-Ras. Note the lack of M-Ras expression in OL. (E) Lysates from DIV5 astrocytes, cortical neurons, and total brain extracts were subjected to Western blotting analysis with antibodies against GFAP and β-III-tubulin. All lysates were probed with an anti-actin antibody to ensure similar protein loading.