FIG. 5.

The WD repeats in WDA are required for the incorporation of WDA into dSAGA. S2 cells were transfected with pRmHa3-wda-HA₂FL₂ and pRmHa3-wdaΔC-HA₂FL₂ plasmids. After a 1-day induction, whole-cell extracts were prepared and incubated with M2-agarose beads. Untransfected S2 cells (WT) were used as negative controls. The immunoprecipitated material was analyzed by Western blotting using antibodies against dGcn5 (rabbit), dSpt3 (rabbit), dAda2B (guinea pig), and FLAG. Lanes 1 to 3 correspond to 40 μg of whole-cell extract (2% input). Lanes 4 to 6 correspond to the immunoprecipitated material (FLAG IP). The arrows show the migration of tagged WDA and WDAΔC. The asterisk points to a cross-reacting band recognized by the anti-FLAG antibodies. α, anti.