FIG. 8.

ypt11Δ cells influence the inheritance of ER membrane markers to a similar extent as observed for myo4Δ cells. (A) Wild-type, myo4Δ, ypt11Δ, and myo4Δ ypt11Δ cells expressing Hmg1p-GFP at the endogenous locus were grown to mid-log phase at 30°C in rich medium and then resuspended in nonfluorescent medium. A z stack image was taken, with the plane going through the bud neck being kept for further investigation. Representative pictures are shown for each genotype. (B) Wild-type, myo4Δ, ypt11Δ, and myo4Δ ypt11Δ cells expressing Sec61p-GFP were photographed as described for panel A. Images were processed as for panel A, with a representative picture shown for each genotype. (C and D) Pictures shown in panel A (C) or B (D) were analyzed using Image J 1.29 software (http://rsb.info.nih.gov/ij). Four sets of data were extracted from the photographs: the intensity signal of the bud cortex, the intensity signal of the mother cortex, the area of the bud, and the area of the mother. Each dot represents one cell and is the ratio of the intensity of the bud cortex/intensity of the mother cortex over the area of the bud/the area of the mother. The experiment was repeated three times, and only one of the experiments is represented in the graph; the tendencies were similar in all three experiments. Table 1 shows a summary quantification of the data set.