FIG. 9.

Functional replacement of SEC19/GDI1 with mammalian Rab-GDI. (A) sec19-1 suppression. sec19-1 cells were transformed with the following plasmids: (1) empty vector, (2) mammalian Rab-GDI, (3) SEC19/GDI1-positive control, as indicated before testing for growth on the permissive and restrictive temperature on yeast-peptone medium containing 1.5% raffinose with 0.5% galactose. (B) Ability of mammalian Rab-GDI constructs to serve as the sole source of yeast SEC19/GDI1. GDI1Δ cells were transformed with constructs as indicated before testing for growth on 5-FOA-containing plates with either glucose or galactose as a carbon source. These constructs are indicated in the plate schematic.