FIG. 3.

DcR1 and DcR2 differentially affect TRAIL DISC formation. (A) Jurkat and (B) HeLa cell populations were stimulated with 5 μg/ml Flag-TRAIL in the presence of 10 μg/ml of M2 cross-linking antibody for the indicated time periods (see Materials and Methods). After cell lysis in NP-40-containing buffer, the DISC was immunoprecipitated (IP) and analyzed by Western blotting using antibodies to DR4, DR5, DcR1, DcR2, FADD, and caspase-8. Caspase-8 cleavage product, corresponding to the p43 fragment, is indicated by black arrowheads. Molecular masses are in kDa. Data are representative of at least three independent experiments.