FIG. 5.

Expression pattern of various neuronal markers in foxl1 MO-injected embryos. Expression of various neuronal markers was examined by whole-mount in situ analysis. Embryos injected with control MO or foxl1 MO were harvested at the indicated stages, and whole-mount in situ hybridization was done. The expression patterns of hlx1, phox2a, and en2 (dorsal view with anterior at the top and a lateral view with dorsal to the right) and of otx2, dlx2, foxb1.2, krox20, and arx (lateral view with anterior to the left) are shown. Embryos with eyes removed are shown in the left and middle panels of hlx1, in the middle panels of en2, and in the panels for the otx2, dlx2, foxb1.2, and Arx samples. The results depicted were obtained with antisense probes. Sense probe control experiments were done for all of the probes, and no significant signals were detected (data not shown). The colored arrowheads are as defined in the text. Abbreviations: pt, pretectum; dt, dorsal thalamus; vt, ventral thalamus; tc, tectum; t, telencephalon; hy, hypothalamus; ot, ocular and trochlear motor progenitors or neurons; c, cranial sensory progenitors or neurons; r, rhombomere. Scale bars, 100 μm.