Figure 4.

Sensitivity of the PS1–Notch1 interaction to SDS. 293 cells were transiently transfected with both FLN6mt and PS1 and either lysed in SDS buffer and analyzed directly (crude lysate) or lysed in coimmunoprecipitation (IP) lysis buffer with the indicated concentration of SDS. The far right sample was also heated for 5 min at 65°C before immunoprecipitation. All samples were then immunoprecipitated with α-PS1_CTF and either boiled (Upper) or heated to 50°C (Lower) before immunoblot. (Upper) The sample was blotted with α-myc. (Lower) The sample was blotted with the anti-PS1 antibody NT1. Sizes of molecular weight markers in kDa are given on the right.