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. 1999 Mar 16;96(6):3269–3274. doi: 10.1073/pnas.96.6.3269

Figure 1.

Figure 1

Effect of CaM-KII on affinity and voltage-dependence of GluR1. (A) Dose–response curves for GluR1 with (squares; n = 4) and without (circles; n = 4) coexpressed CaM-KII. Currents were normalized to those evoked by 10 mM glutamate. (Insert) Whole-cell currents elicited at −80 mV by 10 μM to 30 mM glutamate for GluR1 expressed alone. (B and C Upper) Whole-cell (B) and outside-out patch (C) currents for GluR1 alone elicited by 100-ms pulses of 10 mM glutamate at membrane potentials from −100 mV to 120 mV (20-mV steps). (B and C Lower) Voltage-dependence of whole-cell (B) and outside-out patch (C) currents for GluR1 alone (circles) or for GluR1 coexpressed with CaM-KII (squares). (D) Rectification index for whole-cell (open bars) and outside-out patch (gray bars) currents for GluR1 expressed alone, for GluR1 coexpressed with CaM-KII, for GluR1 with HI CaM-KII infused in the patch pipette, and for Ser-831-to-Glu and Ser-831-to-Asp mutants.