Figure 9.

PI3KC2β-transfected A-431 cells display enhanced proliferation and are resistant to anoikis. (A and B) A-431 and A-431-C2β WT (Myc 6) cells were incubated for 72 h in medium containing 1% serum in the absence (A) or presence (B) of increasing concentrations of a cell-permeable JNK inhibitor peptide. Cell proliferation was assessed using a MTS assay. Data are mean with SD from eight repetitions. (C–E) A-431 and A-431-C2β WT (Myc 6) cells were placed for 12 h (C–E) or 24 h (D) in anchorage-free conditions in the presence or absence of EGF. Cells were then stained using propidium iodide alone (C and D) or in conjunction with annexin V (E) and the proportion of cells in SubG1 (C and D) or positive for annexin V only (E) determined by flow cytometry.