Table 1.
Mitochondrial phenotypes during vegetative growth
| Strain/medium | Mitochondrial morphology (% of cells) | No. of cells scored | ||
|---|---|---|---|---|
| Wild-type–like | Aggregated | Fragmented | ||
| YPD | ||||
| WT | 91.1 | 5.1 | 3.8 | 315 |
| Δmfb1 | 9.4 | 84.7 | 5.9 | 374 |
| Δmdm30 | 10.4 | 88.4 | 1.2 | 346 |
| Δmfb1/Δmdm30 | 1.8 | 83.7 | 14.5 | 339 |
| YPG | ||||
| WT | 84.6 | 0.9 | 14.5 | 331 |
| Δmfb1 | 39.2 | 2.9 | 57.9 | 339 |
| Δmdm30 | 3.7 | 0.6 | 95.7 | 350 |
| Δmfb1/Δmdm30 | 1.3 | 1.3 | 97.4 | 312 |
| YPGal | ||||
| WT/pYES | 90.8 | 3.2 | 6.0 | 532 |
| WT/pYES-MFB1 | 87.5 | 3.0 | 9.5 | 305 |
| WT/pYES-MDM30 | 85.6 | 6.6 | 7.8 | 408 |
| Δmfb1/pYES-MFB1 | 82.8 | 10.5 | 6.7 | 313 |
| Δmdm30/pYES-MDM30 | 84.8 | 7.6 | 7.6 | 422 |
| Δmfb1/pYES | 38.9 | 56.0 | 5.1 | 553 |
| Δmfb1/pYES-MDM30 | 33.5 | 60.8 | 5.7 | 421 |
| Δmdm30/pYES | 6.8 | 86.4 | 6.8 | 308 |
| Δmdm30/pYES-MFB1 | 9.1 | 80.5 | 10.4 | 307 |
| YPD + Cu2+ | ||||
| Δmfb1 | 14.7 | 80.3 | 5.0 | 300 |
| Δmfb1/MFB1 | 69.5 | 27.2 | 3.3 | 305 |
| Δmfb1/MFB1ΔFbox | 78.5 | 20.5 | 1.0 | 302 |
| Δmdm30 | 14.3 | 80.0 | 5.7 | 300 |
| Δmdm30/MDM30 | 48.0 | 48.7 | 3.3 | 304 |
| Δmdm30/MDM30ΔFbox | 59.3 | 38.7 | 2.0 | 300 |
| YPD | ||||
| WT | 99.0 | 0 | 1.0 | 300 |
| cdc53 | 2.5 | 0.5 | 97 | 200 |
| cdc34 | 100.0 | 0 | 0 | 100 |
| pre1 | 97.5 | 0 | 2.5 | 200 |
| YPG | ||||
| WT | 91.5 | 0 | 8.5 | 200 |
| cdc53 | 2.5 | 0 | 97.5 | 200 |
| cdc34 | 78.0 | 0 | 22.0 | 100 |
| pre1 | 90.0 | 0 | 10.0 | 200 |
| YPD + Dox | ||||
| WT | 99.7 | 0.3 | 0 | 300 |
| cdc53 | 4.5 | 3.0 | 92.5 | 200 |
| cdc34 | 14.0 | 0 | 86.0 | 100 |
| pre1 | 24.7 | 0 | 75.3 | 300 |
| YPG + Dox | ||||
| WT | 90.0 | 0 | 10.0 | 200 |
| cdc53 | 3.0 | 2.0 | 95.0 | 100 |
| cdc34 | 24.0 | 0 | 76.0 | 100 |
| pre1 | 31.5 | 0 | 68.5 | 200 |