Thrombin induces translocation to and assembly of the ternary complex components at the PM of endothelial cells. Subconfluent HUVECs stimulated with 10 U/ml thrombin for 30 min (D–F, J–L) or left untreated (A–C, G–I) were labeled for NOSTRIN, eNOS, and caveolin-1 using specific antibodies. Bars, 10 μm.