Figure 6.

Impact of caveolin and cholesterol on ternary complex formation. (A–H) To judge the involvement of caveolin in NOSTRIN-induced translocation of eNOS, CHO-eNOS were infected with SFV-NOSTRIN-GFP (E–H) or left uninfected (A–D). After fixation, cells were stained for caveolin-1 (A and E, red) and eNOS (C and G, blue). Fluorescence of NOSTRIN-GFP (F, green). Triple colocalization is in white. (I–P) To test the impact of the cholesterol membrane distribution on the subcellular localization of the ternary complex, CHO-eNOS cells expressing NOSTRIN-GFP were treated with filipin (I–L) or MβCD (M–P) for 30 min before fixation and specific immunolabeling for caveolin (I and M, red) and eNOS (K and O, blue). Fluorescence of NOSTRIN-GFP (J and N, green). Bars, 10 μm.