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Localization of myosin in sGC mutants. Myosin-GFP was expressed in gc-null, sGCΔcatoe, sGCΔNoe, and sGCoe cells. Cells were placed in the modified Zigmond chemotaxis chamber and were allowed to migrate toward the cAMP. Myosin-GFP fluorescence was visualized by confocal microscopy. The arrow points in the direction of migration.
