Table 1.

Chemotaxis efficiency and degree of turning of mutant sGC cell strains

Cell strain	Spatiotemporal gradient		Early spatial gradient		Late spatial gradient		No. of analyzed cells
	Chemotaxis index	Directional change (°/min)	Chemotaxis index	Directional change (°/min)	Chemotaxis index	Directional change (°/min)
gc-null	0.40 ± 0.04	35.7 ± 1.5	0.37 ± 0.04	37.4 ± 1.6	0.39 ± 0.05	38.4 ± 2.2	85
sGC	0.58 ± 0.03**	28.6 ± 1.5**	0.60 ± 0.04**	27.2 ± 2.0**	0.56 ± 0.06*	31.3 ± 2.5*	63
sGCΔN	0.56 ± 0.03**	31.5 ± 1.2*	0.57 ± 0.04**	30.6 ± 1.2**	0.42 ± 0.04	34.7 ± 1.4	115
sGCΔcat	0.35 ± 0.04	35.0 ± 1.7	0.43 ± 0.04	29.7 ± 1.3**	0.58 ± 0.04**	30.5 ± 1.8**	104
sGCΔNΔcat	0.34 ± 0.05	34.7 ± 3.6	0.40 ± 0.06	36.3 ± 2.6	0.33 ± 005	41.4 ± 5.5	31
sGCΔCΔcat	0.41 ± 0.04	34.8 ± 3.0	0.29 ± 0.07	34.6 ± 4.4	0.27 ± 0.07	36.2 ± 4.0	29

The chemotaxis efficiency and degree of turning of mutant sGC cell strains in the modified Zigmond chemotaxis chamber in response to the spatiotemporal gradient (average of a 4-min window, centered around 6 min after initiation of the gradient), the early spatial gradient (average of a 5-min window at t = 15 min), and the late spatial gradient (average of a 5-min window at t = 30 min) are shown. The degree of turning is defined as follows. For each 1-min step, the displacement of the cells was calculated as a vector. The degree of turning is the angle between subsequent vectors. The data shown are the average and SE of the mean, with n representing the number of cells analyzed. The asterisk(s) indicates that the value is significantly different from the value of gc-null cells at *p < 0.05 or **p < 0.01; data without asterisk(s) are not significantly different at p > 0.05.