Figure 5.

Reduced microtubule dynamicity alters the spatial gradient in tubulin turnover and kinetochore localization. (A) Representative raw GFP-Tub1 FRAP data for a β-tubulin (tub2C254S) mutant from a bin near the SPB (left) and another bin midway between the spindle equator and the SPB (right). Theoretical exponential fit curves calculated based on best-fit half-times by bin are drawn through each plot (solid line). (B) Experimental and simulated GFP-Tub1 FRAP for the β-tubulin mutant with reduced microtubule dynamicity compared with wild-type spindles. The rate of tubulin turnover was decreased in the β-tubulin mutant, especially near the SPB. The position of most rapid FRAP was shifted toward the spindle equator in the β-tubulin mutant (arrows). Simulation parameters and probability-of-fit (p values) for the fit of the experimental data to the simulation are listed in Table 1.