Table 2.

Spindle statistics

Spindle description	GFP-Tub1 FRAP half-time (t1/2; s)a	Spindle length (nm)	XSPB to kinetochore distance (nm)b	Kinetochore cluster spacing (nm)c	Corrected mean kMT length (nm)	n
Wild-type spindles	65 ± 31 (n = 22)	1610 ± 200	430 ± 130	750 ± 280	380 ± 130	79
tub2C354 spindles	146 ± 64 (n = 10)	1530 ± 230	490 ± 90	560 ± 230	440 ± 90	121

Values are mean ± SD.

^a The mean was calculated over a 10-pixel span from the SPB toward the equator.

^b Estimated by multiplying the normalized spindle location of peak mean kinetochore-associated fluorescence by the mean spindle length.

^c Calculated as follows: (mean spindle length) − (2∗SPB to kinetochore distance).