Figure 2.

POD6 is essential for hyphal tip extension. (A) pod-6(31-21) was grown on minimal media plates at 25°C and shifted for 1, 3, and 12 h to restrictive temperature to illustrate the cessation of tip extension with pointed, needle-like tips and the progressive hyperbranching of the mutant. Hyphae that were shifted to the restrictive temperature and then shifted back to the permissive temperature resumed growth at all generated tips within 5 min (center panel). When germinated at the restrictive temperature, pod-6 exhibited a compact, hyperbranched, colonial morphology. (B) Growth of the HP1 pod-6⁺ + HP1 Δpod6 heterokaryotic strain on minimal medium, medium containing fpa and histidine and medium containing benomyl and pantothenic acid (which forces the knockout nucleus to predominate), resulting in morphological defects identical to pod-6(31-21) germinated at or shifted to restrictive temperature.