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. 2006 Sep;17(9):4080–4092. doi: 10.1091/mbc.E06-01-0072

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© 2006 by The American Society for Cell Biology

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Figure 5. — PKA and POD6/COT1 act in parallel pathways. (A) Effect of amending liquid growth media with the PKA inhibitor KT5720. Suppression of the growth defect was monitored after shifting the cultures to restrictive temperatures for 6 h. (B) The growth defect of cot-1(1) and pod-6(31-21) was suppressed in the cr-1 mutant background, whereas (C) mcb;cot-1/pod-6 double mutants are synthetically lethal. For B and C, the strains were grown on minimal media plates supplemented with 1% yeast extract and shifted for the indicated times.