Figure 7.

COT1 and POD6 colocalize and are dependent on microtubule-dependent motor proteins for their correct localization. MYC::COT1 and POD6 were colocalized in growing wild-type hyphae and exhibited punctate/reticulate staining that was evenly distributed throughout the hypha. Note that the Spitzenkörper region at the extreme hyphal apex is unlabeled. (B) This punctate distribution of myc::COT1 and POD6 was altered in dynein heavy chain and conventional kinesin mutants, with myc::COT1 and POD6 accumulating at the hyphal tip in the minus-end-directed (−) ro-1 mutant and in subapical areas/septae in the plus-end-directed (+) nkin mutant. In ro-1, the nuclei are counterstained with DAPI to illustrate that the accumulation at the tip is not due to their clustering, whereas in nkin, septal association of the kinases is indicated by Calcufluor White staining.