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Effect of NCS-1 or KD-PI4Kβ on Akt/PKB phosphorylation. Control, OE-NCS-1, or KD-PI4Kβ cells were IgE-sensitized and activated by DNP-HSA (Ag, 50 ng/ml) for the indicated times. Cells were subsequently lysed, and cell lysates were resolved by SDS-PAGE and immunoblotted with anti-phospho (Ser 473)-Akt antibodies.
