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Structure of a chicken COX-2–luciferase reporter construct. The first 381 nt of a full-length chicken COX-2 cDNA were fused in-frame to the 5′ end of a full-length firefly luciferase cDNA. As shown, the construct encoded the 17-aa, hydrophobic, N-terminal signal peptide (shaded box) of COX-2, as well as its epidermal growth factor (EGF)-like dimerization domain (diagonal stripes) and complete membrane-binding domain (vertical stripes).
