Table 3.

Time course of mitotic and apoptotic responses in Igf1^−/− and wt uterine epithelium

Treatment	MI, %			DI, %			Cell no.			Cell size
	wt	Igf1−/−	P	wt	Igf1−/−	P	wt	Igf1−/−	P	wt	Igf1−/−	P
Vehicle	0.87  ± 0.24	0.22  ± 0.18	0.1	0.42  ± 0.22	0.13  ± 0.08	0.3	264  ± 13	247  ± 46	0.73	9.2  ± 1.5 μ	6.8  ± 0.4 μ	0.17
21 h post-E2	16.03  ± 1.03	4.64  ± 1.26	0.0001	1.21  ± 0.16	0.50  ± 0.08	0.44	400  ± 49	230  ± 19	0.003	16.5  ± 1.2 μ	10.5  ± 1.0 μ	0.003
48 h post-E2	1.19  ± 0.18	4.60  ± 0.72	0.015	80.33  ± 6.3	2.08  ± 0.75	0.0001	492  ± 78	357  ± 28	0.055	17.1  ± 13 μ	14.6  ± 1.0 μ	0.19

The mitotic index (MI) and death index (DI) were obtained by scoring the number of mitotic and apoptotic figures per 100 epithelial nuclei in hemotoxylinleosin-stained uterine sections. Evaluation of mitotic and apoptotic figures by DAPI (4,6-diamidino-2-phenylindole) staining confirmed the findings with hemotoxylinleosin. The cell number data represents the number of luminal epithelial cells present in anatomically matched uterine cross sections. The cell size data represents the length of luminal epithelial cells from apical to basement membranes. All data are mean ± SEM, with 3–12 mice in each group.