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Guanidine stimulates oligomerization of AβMRF in the absence of HSP104. AβMRF-expressing sup35Δ hsp104Δ cells were grown in the absence (-) or presence (+) of 6.3 mM guanidine (Gu). Equal amounts of lysate proteins were treated with 1% SDS and analyzed by immunoblotting with anti-RF antibodies following electrophoresis in agarose. Equal protein loading was confirmed by coomassie staining of the membrane (not shown).
