TABLE 6.
The core exoproteome of S. aureus, defined as proteins with predicted Sec-type signal peptides present in all sequenced strainsl
| Identification no. | Protein | Amino acid at position:
|
Function | |||
|---|---|---|---|---|---|---|
| −3 | −2 | −1 | +1 | |||
| 15925728a,b | SA0022 | S | N | A | A | Hypothetical protein |
| 15925815a | Spa | A | N | A | A | Immunoglobulin G binding protein A precursor |
| 15925838c | SA0129 | A | H | A | D | Hypothetical protein |
| 15925848d | SA0139 | S | L | A | I | Hypothetical protein |
| 15925933 | Coa | A | D | A | I | Staphylocoagulase precursor |
| 15925978 | LytM | A | D | A | A | Peptidoglycan hydrolase |
| 15926008 | SA0295 | A | F | A | K | Hypothetical protein |
| 15926022d | Geh | A | Q | A | S | Glycerol ester hydrolase |
| 15926106e | Set13 | V | H | A | K | Exotoxin 13 |
| 15926112 | SA0394 | A | E | A | S | Hypothetical protein |
| 15926142d,f | SA0423 | A | N | A | A | Hypothetical protein |
| 15926239a,d | SdrC | A | K | A | A | Ser-Asp-rich fibrinogen-binding protein |
| 15926291d | SA0570 | A | E | A | A | Hypothetical protein |
| 15926319d,g,h | Pbp4 | A | Q | A | T | Penicillin binding protein 4 |
| 15926342f,d | SA0620 | A | Q | A | S | Secretory antigen SsaA homologue |
| 15926373d | SA0651 | A | L | A | K | Hypothetical protein |
| 15926432f,d | SA0710 | A | H | A | Q | Hypothetical protein |
| 15926464a | ClfA | A | D | A | S | Fibrinogen-binding protein A |
| 15926466 | Ssp | A | K | A | A | Extracellular matrix and plasma binding protein |
| 15926467 | SA0745 | A | N | A | L | Hypothetical protein |
| 15926548h | GlpQ | A | G | A | E | Glycerophosphoryl diester phosphodiesterase |
| 15926570 | SA0841 | V | S | A | A | Hypothetical protein |
| 15926634 | SspB | A | K | A | D | Cysteine protease precursor |
| 15926635d | SspA | A | N | A | L | Serine protease |
| 15926639f,d | Atl | V | Q | A | A | Autolysin |
| 15926713a | IsdB | A | Q | A | A | Hypothetical protein |
| 15926714a | IsdA | V | N | A | A | Cell surface protein |
| 15926715i | IsdC | A | N | A | A | Hypothetical protein |
| 15926738 | SA1000 | S | H | A | Q | Hypothetical protein |
| 15926741 | Efb | A | D | A | S | Hypothetical protein |
| 15926742 | SA1004 | A | D | A | S | Hypothetical protein |
| 15926750 | SA1010 | S | E | A | K | Hypothetical protein |
| 15926830f | LytN | A | Y | A | D | LytN protein |
| 15927055d | GpsA | V | L | A | E | Glycerol-3-phosphate dehydrogenase |
| 15927385 | SplC | A | N | A | E | Serine protease SplC |
| 15927456d | SA1698 | S | L | A | D | Hypothetical protein |
| 15927483d | SspB2 | A | N | A | E | Staphopain, cysteine proteinase |
| 15927580 | SA1812 | S | Y | A | K | Hypothetical protein |
| 15927607d | SA1839† | V | E | A | K | Hypothetical protein |
| 15927670d | SA1898 | A | H | A | S | Hypothetical protein |
| 15927785 | SA2006 | A | S | A | D | Hypothetical protein |
| 15927879d | SsaA | A | H | A | S | Hypothetical protein |
| 15927884d | SA2097 | A | D | A | A | Hypothetical protein |
| 15927996 | Sbi | A | K | A | S | IgG-binding protein Sbi |
| 15927997 | HlgA | S | K | A | E | γ-Hemolysin chain II precursor |
| 15927998 | HlgC | A | K | A | A | γ-Hemolysin component C |
| 15927999 | HlgB | A | N | A | E | γ-Hemolysin component B |
| 15928114 | SA2323 | A | Y | A | H | Hypothetical protein |
| 15928123d | SA2332 | S | H | A | A | Hypothetical protein |
| 15928145d | SA2353 | A | Q | A | A | Hypothetical protein |
| 15928148d | IsaA | A | H | A | A | Immunodominant antigen A |
| 15928216a | ClfB | A | Q | A | S | Clumping factor B |
| 15928223d,j | Aur | A | L | A | I | Zinc metalloproteinase aureolysin |
| 15928224d,k | IsaB | A | Q | A | A | Immunodominant antigen B |
| 15928230d | SA2437 | A | Y | A | D | Hypothetical protein |
| 15928232c,d | SasF | A | Q | A | A | Conserved hypothetical protein |
| 15928254 | IcaB | A | N | A | D | Intercellular adhesion protein B |
| 15928257d | Lip | A | Q | A | A | Triacylglycerol lipase precursor |
Contains an LPXTG motif.
Protein homologue of B. subtilis is found in extracellular proteome (174).
Contains an LPXAG motif.
Also present in S. epidermidis.
Only the S. aureus COL protein has a Thr at the +1 position, which is not included in the recognition and cleavage pattern. All other S. aureus strains conform to the proposed pattern.
Contains a LysM or GW domain motif.
Only the S. aureus N315 protein has a Thr at the +1 position, which is not included in the recognition and cleavage pattern. All other S. aureus strains conform to the proposed pattern.
Protein homologues of B. subtilis are classified as Sec-attached membrane proteins (179).
Contains an NPQTN motif.
Protein homologue of B. subtilis classified as a secretory protein (179).
Only for the S. aureus MRSA252 protein; protein homologues in other strains are predicted to have two transmembrane domains and were excluded from the list.
Signal peptide predictions were performed with SignalP-NN and SignalP-HMM, version 2.0 (136; http://www.cbs.dtu.dk/services/SignalP-2.0/), PrediSi (76; http://www.predisi.de/), Phobius (95; http://phobius.cgb.ki.se/), and LipoP, version 1.0 (94; http://www.cbs.dtu.dk/services/LipoP/). These programs are designed to identify Sec-type signal peptides, amino-terminal membrane anchors (Phobius), or lipoprotein signal peptides in gram-negative bacteria (LipoP). The TMHMM program, version 2.0 (41; http://www.cbs.dtu.dk/services/TMHMM/), was used to identify transmembrane segments in proteins.