TABLE 2.

Synthetic oligonucleotide primers used for set allotyping by PCR

Gene targeted	Primer sequences (forward; reverse)a	Annealing temp (°C)	Amplicon size (bp)
set2	5′-CAACAGARGYYAATTCAGGTC-3′;	52	499
	5′-RAYCCTACCATCTTTTGAGAK-3′
set5	5′-CGATACTACTCAKCASMAAG-3′;	52	511
	5′-TATTCAACACATCGCCCATG-3′
set7	5′-TGCGAGYGAACATKAAGCAA-3′;	52	573
	5′-SRYRTCACYCATRCGRTKWG-3′

^aR, G/A; Y, C/T; K, G/T; S, G/C; W, T/A; M, A/C.