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Sensitivity of VP1 RT-snPCR and comparison of the sensitivity with that of the 5′ nontranslated region RT-snPCR. (A) Amplification of RNA extracted from 10-fold serial dilutions of an EV68 virus stock; (B) amplification of 10-fold serial dilutions of VP3-VP1 sRNA; (C) comparison of VP1 RT-snPCR (top) with 5′-NTR RT-snPCR (bottom) using 10-fold serial dilutions.
