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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of expression and purification of recombinant DV1 NS1 protein from Escherichia coli. Lane 1, uninduced crude cell lysates; lanes 2 to 5, induced crude cell lysates under different conditions; lanes 6 to 9, recombinant DV1 NS1 protein purified by Ni-nitrilotriacetic acid affinity chromatography; M, markers with their corresponding molecular masses.
