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Control dot blot analysis of protein extracts from uninfected red blood cells (RBC) and two P. falciparum isolates. Protein extracts were prepared at a dilution of 1/8 before application onto each membrane, as indicated at the bottom of each row. Membranes were then incubated at a dilution of 1/2,000 with the four monoclonal antibodies 4A5, 3A4, 2G12-1C12, and 1E1-A9.
